{"id":305,"date":"2014-01-02T00:28:27","date_gmt":"2014-01-02T04:28:27","guid":{"rendered":"http:\/\/jimlund.org\/blog\/?p=305"},"modified":"2014-01-02T00:28:27","modified_gmt":"2014-01-02T04:28:27","slug":"abi-377-teardown","status":"publish","type":"post","link":"http:\/\/jimlund.org\/blog\/?p=305","title":{"rendered":"ABI 377 Teardown"},"content":{"rendered":"<p>I picked up two ABI PRISM 377 DNA sequencers.  These are the last generation of slab gel sequencers.<br \/>\n<a href=\"pics\/20131222_201725.jpg\"><img decoding=\"async\" src=\"pics\/20131222_201725.2.jpg\" alt=\"ABI 377\" \/><\/a><\/p>\n<p><a href=\"pics\/20131222_203218.jpg\"><img decoding=\"async\" src=\"pics\/20131222_203218.2.jpg\" alt=\"ABI 377\" \/><\/a><\/p>\n<p>With the front open, you can see the place where the gel gets mounted.<\/p>\n<p><a href=\"pics\/20131222_201740.jpg\"><img decoding=\"async\" src=\"pics\/20131222_201740.2.jpg\" alt=\"gel door open\" \/><\/a><\/p>\n<p>The left side opens, and the bottom cover comes off.  The laser can be seen at the bottom, and some of the power supplies on the left.<\/p>\n<p><a href=\"pics\/20131222_201810.jpg\"><img decoding=\"async\" src=\"pics\/20131222_201810.2.jpg\" alt=\"Open cabinet\" \/><\/a><\/p>\n<p>Here is the laser, a Uniphase Argon laser, 0.5W 2214-40MLA 1998.<\/p>\n<p><a href=\"pics\/20131222_202021.jpg\"><img decoding=\"async\" src=\"pics\/20131222_202021.2.jpg\" alt=\"laser\" \/><\/a><br \/>\n<a href=\"pics\/20131222_202120.jpg\"><img decoding=\"async\" src=\"pics\/20131222_202120.2.jpg\" alt=\"laser\" \/><\/a><\/p>\n<p>The power supply modules are located on the left side.  On the top left is the laser power supply.  The electrophoresis power supply is on the left in the middle.  To the right of it is the power distribution center&#8211;plugs for the laser and electrophoresis power supplies, and the blower motor.  On the bottom at the left is the blower motor.  In the middle at the bottom the top of a mirror module that bounces the laser back to the right at the level of the bottom of the gel.  On the right at the bottom, the servo motor that moves the detector unit along the bottom of the gel.<\/p>\n<p><a href=\"pics\/20131222_201914.jpg\"><img decoding=\"async\" src=\"pics\/20131222_201914.2.jpg\" alt=\"left side\" \/><\/a><\/p>\n<p>Here&#8217;s the laser power supply.  The laser is not plugged in.<br \/>\n<a href=\"pics\/20131222_201834.jpg\"><img decoding=\"async\" src=\"pics\/20131222_201834.2.jpg\" alt=\"laser power supply\" \/><\/a><\/p>\n<p>Close up of the laser power supply, Uniphase 2114B-40MLA 12A:<br \/>\n<a href=\"pics\/20131222_202140.jpg\"><img decoding=\"async\" src=\"pics\/20131222_202140.2.jpg\" alt=\"laser power supply\" \/><\/a><\/p>\n<p>The power distribution center labels: J41 DC Power Supply  Max 4000W J40 Heater and Pump Control<\/p>\n<p>The electrophoresis power supply: Spellman P\/N X2094 Rev. E4 Model No PTV5P300X2094<br \/>\n230V 5A, output 0-5kV, 0-60mA.<\/p>\n<p>Here is a closeup of the servo: Telcomm brushless servo motor<br \/>\n<a href=\"pics\/20131222_203946.jpg\"><img decoding=\"async\" src=\"pics\/20131222_203946.2.jpg\" alt=\"servo\" \/><\/a><\/p>\n<p>On the back side of the machine behind the top panel is this circuit board, the control, data processing, and interface board.<\/p>\n<p><a href=\"pics\/20131222_203246.jpg\"><img decoding=\"async\" src=\"pics\/20131222_203246.2.jpg\" alt=\"main board\" \/><\/a><\/p>\n<p>Two interesting chips on the board, a FPGA and a pair of voltage converters.<\/p>\n<p><a href=\"pics\/20131222_203304.jpg\"><img decoding=\"async\" src=\"pics\/20131222_203304.2.jpg\" alt=\"xilinx\" \/><\/a><br \/>\nThe FPGA is a <a href=\"pics\/xilinx_xc3064a.pdf\">Xilinx XC3064A<\/a><\/p>\n<p>The voltage converter.<br \/>\n<a href=\"pics\/20131222_203337-2.jpg\"><img decoding=\"async\" src=\"pics\/20131222_203337-2.2.jpg\" alt=\"voltage converter\" \/><\/a><\/p>\n<p>On the left of the main board is region with cooling lines:<br \/>\n<a href=\"pics\/20131222_203345.jpg\"><img decoding=\"async\" src=\"pics\/20131222_203345.2.jpg\" alt=\"pump area\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>I picked up two ABI PRISM 377 DNA sequencers. These are the last generation of slab gel sequencers. With the front open, you can see the place where the gel gets mounted. The left side opens, and the bottom cover comes off. The laser can be seen at the bottom, and some of the power [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2,7,14],"tags":[],"class_list":["post-305","post","type-post","status-publish","format-standard","hentry","category-biotech","category-hobbies","category-science"],"_links":{"self":[{"href":"http:\/\/jimlund.org\/blog\/index.php?rest_route=\/wp\/v2\/posts\/305","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/jimlund.org\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/jimlund.org\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/jimlund.org\/blog\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/jimlund.org\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=305"}],"version-history":[{"count":0,"href":"http:\/\/jimlund.org\/blog\/index.php?rest_route=\/wp\/v2\/posts\/305\/revisions"}],"wp:attachment":[{"href":"http:\/\/jimlund.org\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=305"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/jimlund.org\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=305"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/jimlund.org\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=305"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}