November 3rd, 2018
Chicago police search arrests
Chicago Police Department BAIL BOND MANUAL
Shetland sheep
“A Shetland ram was kept by United States President Thomas Jefferson for several years in the early nineteenth century. Unlike modern Shetlands (but like some related breeds) this ram had four horns. He was kept with about 40 other sheep on President’s Square in front of the White House. In the spring of 1808, it attacked several people who had taken shortcuts across the square, injuring some and actually killing a small boy.[8] Having been moved to Jefferson’s private estate at Monticello, the ram was eventually killed after having killed several other rams: it was described by Jefferson as “this abominable animal”.[8] Such aggressive Shetland rams, however, are unusual.[9]”
Candice Delmas, A Duty to Resist: When Disobedience Should Be Uncivil (2018)
QAnon’s true believers are devastated as the conspiracy theory goes down in flames
How to Stock a Biology/Genetics Lab
Justin Teng’s Personal Go Book Collection and Guide
Timothy Denevi, author of “Freak Kingdom,” details how Hunter S. Thompson “is really good at trying to see what’s necessary and not, in terms of justice.” by Erin Keane
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October 24th, 2018
STAR-Fusion is a program that detects RNA fusions events in RNA-Seq data. According to the paper describing the program, STAR-Fusion is much better than the dozen or so other callers under active development.
Still, reading the paper left me with some questions. As descried by the authors, STAR-Fusion is not just a good caller, but the best caller by a wide margin. See Fig 3A. The next nine best callers have AUC values of 0.5 to 0.3, but STAR-Fusion has a value of 0.8 in the author’s testing.
And what is the source of this incredible result? The authors are silent on the subject. They don’t know, or perhaps didn’t notice how remarkable their achievement is, and so don’t remark on it. The description of the STAR-Fusion algorithm seems very similar to the algorithms used by every other RNA fusion caller. Some do better than others, so details of implementation must matter.
So what is the critical advance STAR-Fusion makes? Is better sequence alignment key? Is the filtering approach? The paralog handling seems like it cuts down on false positives, is this key? Discovering the critical factors for RNA fusion calling would be an important result.
Or are the performance results in the paper dependent on the synthetic test data set the authors use? Will subsequent papers comparing STAR-Fusion to other methods find that it is only average, or sub-par?
Posted in biotech, Software | No Comments »
October 11th, 2018
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September 9th, 2018
Thermo Nicolet IR200 Spectrometer investigation
Requires a custom power brick, multiple pins. Could not find power specifications. Power bricks do not seem to be available.
Laser was removed from this unit. This is a HeNe laser used “provies the reference signal for triggering data collections and measuring the stroke of the moving mirror.”
Dessicant spot indicates that the dessicant was exhausted. Dessicant appears to be a standard pack. Recharged dessicant in 250F oven for three hours. I’ll try to preserve the optics, I may be able to reuse them.
Requires ENCOMPASS or OMNIC software. I was able to find an OMNIC iso. Untested.
“The interferometer window in your spectrometer is made of KRS-5…” KRS-5 is thallium bromoiodide (TlBr-TlI). KRs-5 (TlBr-TlI) is a gorgeous red crystal commonly used for attenuated total reflection prisms for IR spectroscopy. It is also used as an infrared transmission window in gas and liquid sample cells used with FTIR spectrophotometers in place of Potassium Bromide (KBr) or Cesium Iodide (CsI) for analysis of aqueous samples that would attack KBr or CsI optics. It has a wide transmission range and is virtually insoluble in water. It is a useful alternative to AgCl since it is not photo-sensitive and for ATR applications it will transmit well beyond the 18 micron useful range of ZnSe.
Background on FT-IR:
wikipedia
Nicolet brochure
Unit with case removed:
IR mirror on the left. The right side module looks like a detector:
Back of detector module:
Dry optics compartment:
Dry optics compartment:
Laser diode?:
Corner mirror and flat mirror:
Second corner mirror on solenoid:
Nicolet IR100 and Nicolet IR200 User’s Guide
Nicolet 4700 or Nicolet 6700 User’s Guide
OMNIC User’s Guide, v7.4Transport Kit User’s Guide
Also have “OMNIC- for TIR.iso”
Could not find a service manual.
Posted in Uncategorized | 6 Comments »
August 10th, 2018
After the 2000 ‘hanging chad’ election, the Election Commission report concluded that about 10% of votes are never counted or miss-counted. The Republican President and House didn’t much care, threw a few billion at the states some counties replaced the punch card machines w/ electronic voting machines. It made things worse. The election results are still untrustworthy, but in many places there’s no paper record to check but there is a poorly designed and poorly secured software program counting the votes. And yet worse, these voting machine companies are all private, and keep their software a secret, so there is no way to audit the software or check the election results. In the *rare* cases where security researchers have inspected the software and computerized voting machines, they have always proved easy to break into and subvert. Same with the centralized websites counting and recording the votes. Complete failure of democracy.
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August 6th, 2018
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July 8th, 2018
Don’t Talk to Cops. Mr. James Duane, a professor at Regent Law School and a former defense attorney, tells you why you should never agree to be interviewed by the police.
10 Rules for Dealing with Police
The RIGHT Way to Handle a Police Stop
A feminist Analysis of Die Hard
Start Making Children Happy With Math
Mandelbrot Fractals
10 Ways to Cover a Trump Rally
John Kenneth Galbraith (1963): Wealth and Poverty: “The modern conservative… not even especially modern… is engaged… in one of man’s oldest, best financed, most applauded, and, on the whole, least successful exercises in moral philosophy. That is the search for a superior moral justification for selfishness… link
Warriors books
Megatons To Megawatts: Russian Warheads Fuel U.S. Power Plants
First World Problems, BBC
Scanner frequencies for Chicago, 1, 2, 3
Baofeng UV-5R info
Stability of Active Ingredients in Long-Expired Prescription Medications
Stupid Rightwing memes: “The United States is not a ‘democracy,’ it is ‘a Republic.'”. I don’t quite get the point of this one. Impress the rubes?
Bullseye Bore–laser circle shows if drill is held at 90 degrees
West Nile virus incidence
CCR5Δ32 allele common in Europeans, loss prevents HIV infections, makes carriers more susceptible to West Nile
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